normal human epidermal keratinocytes from neonatal foreskin Search Results


neonatal human epidermal keratinocytes  (ATCC)
99
ATCC neonatal human epidermal keratinocytes
Neonatal Human Epidermal Keratinocytes, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/neonatal human epidermal keratinocytes/product/ATCC
Average 99 stars, based on 1 article reviews
neonatal human epidermal keratinocytes - by Bioz Stars, 2026-06
99/100 stars
  Buy from Supplier
primary normal human epidermal keratinocytes  (ATCC)
94
ATCC primary normal human epidermal keratinocytes
BRD4 and p63 regulates <t>keratinocytes</t> proliferation and differentiation. a, b, e, f Cell cycle analysis performed in HEKn comparing SCR condition and sip63 and siBRD4 conditions comparing untreated cells (DMSO) and JQ1 treated cells (10 µM). Cells were stained with Propidium Iodide (50 µg/ml) for 1 h and then analysed by flow cytometry. In a, e representative plot of cell cycle analysis performed in HEKn is shown, while in b, f a quantification of cell cycle analysis. Graphs present means ± SD of three independent experiments. c, g EdU-incorporation assay of HEKn cells transfected with siSCR, sip63 and different BRD4 (siBRD4#1, BRD4#2) siRNAs or treated with DMSO and JQ1 (10 µM). Data are shown as mean ± SD of N = 3 experiments. (Unpaired Student’s t test). d, h Growth curve of HEKn cells transfected with siSCR, sip63 and siBRD4#1, or treated with DMSO and JQ1 (10 µM). The cell confluency has been determined using the Incucyte real-time video imaging system. Each data points indicate mean ± SEM. i – n Western blot analysis was carried out with specific antibodies against K10, and β-actin was used as loading control. ImageJ program was used to quantitate the protein levels. The blot is representative of three independent experiments. DD, days of differentiation
Primary Normal Human Epidermal Keratinocytes, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/primary normal human epidermal keratinocytes/product/ATCC
Average 94 stars, based on 1 article reviews
primary normal human epidermal keratinocytes - by Bioz Stars, 2026-06
94/100 stars
  Buy from Supplier
single-donor, neonatal-foreskin tissue-derived normal human epidermal keratinocyte and fibroblast 3d cultures  (MatTek)
90
MatTek single-donor, neonatal-foreskin tissue-derived normal human epidermal keratinocyte and fibroblast 3d cultures
BRD4 and p63 regulates <t>keratinocytes</t> proliferation and differentiation. a, b, e, f Cell cycle analysis performed in HEKn comparing SCR condition and sip63 and siBRD4 conditions comparing untreated cells (DMSO) and JQ1 treated cells (10 µM). Cells were stained with Propidium Iodide (50 µg/ml) for 1 h and then analysed by flow cytometry. In a, e representative plot of cell cycle analysis performed in HEKn is shown, while in b, f a quantification of cell cycle analysis. Graphs present means ± SD of three independent experiments. c, g EdU-incorporation assay of HEKn cells transfected with siSCR, sip63 and different BRD4 (siBRD4#1, BRD4#2) siRNAs or treated with DMSO and JQ1 (10 µM). Data are shown as mean ± SD of N = 3 experiments. (Unpaired Student’s t test). d, h Growth curve of HEKn cells transfected with siSCR, sip63 and siBRD4#1, or treated with DMSO and JQ1 (10 µM). The cell confluency has been determined using the Incucyte real-time video imaging system. Each data points indicate mean ± SEM. i – n Western blot analysis was carried out with specific antibodies against K10, and β-actin was used as loading control. ImageJ program was used to quantitate the protein levels. The blot is representative of three independent experiments. DD, days of differentiation
Single Donor, Neonatal Foreskin Tissue Derived Normal Human Epidermal Keratinocyte And Fibroblast 3d Cultures, supplied by MatTek, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/single-donor, neonatal-foreskin tissue-derived normal human epidermal keratinocyte and fibroblast 3d cultures/product/MatTek
Average 90 stars, based on 1 article reviews
single-donor, neonatal-foreskin tissue-derived normal human epidermal keratinocyte and fibroblast 3d cultures - by Bioz Stars, 2026-06
90/100 stars
  Buy from Supplier
normal human neonatal foreskin epidermal keratinocytes (nhek)  (Kurabo industries)
90
Kurabo industries normal human neonatal foreskin epidermal keratinocytes (nhek)
BRD4 and p63 regulates <t>keratinocytes</t> proliferation and differentiation. a, b, e, f Cell cycle analysis performed in HEKn comparing SCR condition and sip63 and siBRD4 conditions comparing untreated cells (DMSO) and JQ1 treated cells (10 µM). Cells were stained with Propidium Iodide (50 µg/ml) for 1 h and then analysed by flow cytometry. In a, e representative plot of cell cycle analysis performed in HEKn is shown, while in b, f a quantification of cell cycle analysis. Graphs present means ± SD of three independent experiments. c, g EdU-incorporation assay of HEKn cells transfected with siSCR, sip63 and different BRD4 (siBRD4#1, BRD4#2) siRNAs or treated with DMSO and JQ1 (10 µM). Data are shown as mean ± SD of N = 3 experiments. (Unpaired Student’s t test). d, h Growth curve of HEKn cells transfected with siSCR, sip63 and siBRD4#1, or treated with DMSO and JQ1 (10 µM). The cell confluency has been determined using the Incucyte real-time video imaging system. Each data points indicate mean ± SEM. i – n Western blot analysis was carried out with specific antibodies against K10, and β-actin was used as loading control. ImageJ program was used to quantitate the protein levels. The blot is representative of three independent experiments. DD, days of differentiation
Normal Human Neonatal Foreskin Epidermal Keratinocytes (Nhek), supplied by Kurabo industries, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/normal human neonatal foreskin epidermal keratinocytes (nhek)/product/Kurabo industries
Average 90 stars, based on 1 article reviews
normal human neonatal foreskin epidermal keratinocytes (nhek) - by Bioz Stars, 2026-06
90/100 stars
  Buy from Supplier
normal human neonatal epidermal keratinocyte progenitor cells lot#eb1110044 (pooled)  (CELLnTEC Advanced Cell Systems AG)
90
CELLnTEC Advanced Cell Systems AG normal human neonatal epidermal keratinocyte progenitor cells lot#eb1110044 (pooled)
BRD4 and p63 regulates <t>keratinocytes</t> proliferation and differentiation. a, b, e, f Cell cycle analysis performed in HEKn comparing SCR condition and sip63 and siBRD4 conditions comparing untreated cells (DMSO) and JQ1 treated cells (10 µM). Cells were stained with Propidium Iodide (50 µg/ml) for 1 h and then analysed by flow cytometry. In a, e representative plot of cell cycle analysis performed in HEKn is shown, while in b, f a quantification of cell cycle analysis. Graphs present means ± SD of three independent experiments. c, g EdU-incorporation assay of HEKn cells transfected with siSCR, sip63 and different BRD4 (siBRD4#1, BRD4#2) siRNAs or treated with DMSO and JQ1 (10 µM). Data are shown as mean ± SD of N = 3 experiments. (Unpaired Student’s t test). d, h Growth curve of HEKn cells transfected with siSCR, sip63 and siBRD4#1, or treated with DMSO and JQ1 (10 µM). The cell confluency has been determined using the Incucyte real-time video imaging system. Each data points indicate mean ± SEM. i – n Western blot analysis was carried out with specific antibodies against K10, and β-actin was used as loading control. ImageJ program was used to quantitate the protein levels. The blot is representative of three independent experiments. DD, days of differentiation
Normal Human Neonatal Epidermal Keratinocyte Progenitor Cells Lot#Eb1110044 (Pooled), supplied by CELLnTEC Advanced Cell Systems AG, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/normal human neonatal epidermal keratinocyte progenitor cells lot#eb1110044 (pooled)/product/CELLnTEC Advanced Cell Systems AG
Average 90 stars, based on 1 article reviews
normal human neonatal epidermal keratinocyte progenitor cells lot#eb1110044 (pooled) - by Bioz Stars, 2026-06
90/100 stars
  Buy from Supplier
cdna prepared from epidermal keratinocytes of human neonates  (Kurabo industries)
90
Kurabo industries cdna prepared from epidermal keratinocytes of human neonates
BRD4 and p63 regulates <t>keratinocytes</t> proliferation and differentiation. a, b, e, f Cell cycle analysis performed in HEKn comparing SCR condition and sip63 and siBRD4 conditions comparing untreated cells (DMSO) and JQ1 treated cells (10 µM). Cells were stained with Propidium Iodide (50 µg/ml) for 1 h and then analysed by flow cytometry. In a, e representative plot of cell cycle analysis performed in HEKn is shown, while in b, f a quantification of cell cycle analysis. Graphs present means ± SD of three independent experiments. c, g EdU-incorporation assay of HEKn cells transfected with siSCR, sip63 and different BRD4 (siBRD4#1, BRD4#2) siRNAs or treated with DMSO and JQ1 (10 µM). Data are shown as mean ± SD of N = 3 experiments. (Unpaired Student’s t test). d, h Growth curve of HEKn cells transfected with siSCR, sip63 and siBRD4#1, or treated with DMSO and JQ1 (10 µM). The cell confluency has been determined using the Incucyte real-time video imaging system. Each data points indicate mean ± SEM. i – n Western blot analysis was carried out with specific antibodies against K10, and β-actin was used as loading control. ImageJ program was used to quantitate the protein levels. The blot is representative of three independent experiments. DD, days of differentiation
Cdna Prepared From Epidermal Keratinocytes Of Human Neonates, supplied by Kurabo industries, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cdna prepared from epidermal keratinocytes of human neonates/product/Kurabo industries
Average 90 stars, based on 1 article reviews
cdna prepared from epidermal keratinocytes of human neonates - by Bioz Stars, 2026-06
90/100 stars
  Buy from Supplier
hek lonza nhek-neo-neonatal normal human epidermal keratinocytes, pooled  (Lonza)
90
Lonza hek lonza nhek-neo-neonatal normal human epidermal keratinocytes, pooled
BRD4 and p63 regulates <t>keratinocytes</t> proliferation and differentiation. a, b, e, f Cell cycle analysis performed in HEKn comparing SCR condition and sip63 and siBRD4 conditions comparing untreated cells (DMSO) and JQ1 treated cells (10 µM). Cells were stained with Propidium Iodide (50 µg/ml) for 1 h and then analysed by flow cytometry. In a, e representative plot of cell cycle analysis performed in HEKn is shown, while in b, f a quantification of cell cycle analysis. Graphs present means ± SD of three independent experiments. c, g EdU-incorporation assay of HEKn cells transfected with siSCR, sip63 and different BRD4 (siBRD4#1, BRD4#2) siRNAs or treated with DMSO and JQ1 (10 µM). Data are shown as mean ± SD of N = 3 experiments. (Unpaired Student’s t test). d, h Growth curve of HEKn cells transfected with siSCR, sip63 and siBRD4#1, or treated with DMSO and JQ1 (10 µM). The cell confluency has been determined using the Incucyte real-time video imaging system. Each data points indicate mean ± SEM. i – n Western blot analysis was carried out with specific antibodies against K10, and β-actin was used as loading control. ImageJ program was used to quantitate the protein levels. The blot is representative of three independent experiments. DD, days of differentiation
Hek Lonza Nhek Neo Neonatal Normal Human Epidermal Keratinocytes, Pooled, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/hek lonza nhek-neo-neonatal normal human epidermal keratinocytes, pooled/product/Lonza
Average 90 stars, based on 1 article reviews
hek lonza nhek-neo-neonatal normal human epidermal keratinocytes, pooled - by Bioz Stars, 2026-06
90/100 stars
  Buy from Supplier
neonatal normal human epidermal keratinocyte cells cc-2503  (Lonza)
90
Lonza neonatal normal human epidermal keratinocyte cells cc-2503
BRD4 and p63 regulates <t>keratinocytes</t> proliferation and differentiation. a, b, e, f Cell cycle analysis performed in HEKn comparing SCR condition and sip63 and siBRD4 conditions comparing untreated cells (DMSO) and JQ1 treated cells (10 µM). Cells were stained with Propidium Iodide (50 µg/ml) for 1 h and then analysed by flow cytometry. In a, e representative plot of cell cycle analysis performed in HEKn is shown, while in b, f a quantification of cell cycle analysis. Graphs present means ± SD of three independent experiments. c, g EdU-incorporation assay of HEKn cells transfected with siSCR, sip63 and different BRD4 (siBRD4#1, BRD4#2) siRNAs or treated with DMSO and JQ1 (10 µM). Data are shown as mean ± SD of N = 3 experiments. (Unpaired Student’s t test). d, h Growth curve of HEKn cells transfected with siSCR, sip63 and siBRD4#1, or treated with DMSO and JQ1 (10 µM). The cell confluency has been determined using the Incucyte real-time video imaging system. Each data points indicate mean ± SEM. i – n Western blot analysis was carried out with specific antibodies against K10, and β-actin was used as loading control. ImageJ program was used to quantitate the protein levels. The blot is representative of three independent experiments. DD, days of differentiation
Neonatal Normal Human Epidermal Keratinocyte Cells Cc 2503, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/neonatal normal human epidermal keratinocyte cells cc-2503/product/Lonza
Average 90 stars, based on 1 article reviews
neonatal normal human epidermal keratinocyte cells cc-2503 - by Bioz Stars, 2026-06
90/100 stars
  Buy from Supplier
prewounded, single-donor, neonatal-foreskin tissue-derived normal human epidermal keratinocyte and fibroblast 3d cultures  (MatTek)
90
MatTek prewounded, single-donor, neonatal-foreskin tissue-derived normal human epidermal keratinocyte and fibroblast 3d cultures
BRD4 and p63 regulates <t>keratinocytes</t> proliferation and differentiation. a, b, e, f Cell cycle analysis performed in HEKn comparing SCR condition and sip63 and siBRD4 conditions comparing untreated cells (DMSO) and JQ1 treated cells (10 µM). Cells were stained with Propidium Iodide (50 µg/ml) for 1 h and then analysed by flow cytometry. In a, e representative plot of cell cycle analysis performed in HEKn is shown, while in b, f a quantification of cell cycle analysis. Graphs present means ± SD of three independent experiments. c, g EdU-incorporation assay of HEKn cells transfected with siSCR, sip63 and different BRD4 (siBRD4#1, BRD4#2) siRNAs or treated with DMSO and JQ1 (10 µM). Data are shown as mean ± SD of N = 3 experiments. (Unpaired Student’s t test). d, h Growth curve of HEKn cells transfected with siSCR, sip63 and siBRD4#1, or treated with DMSO and JQ1 (10 µM). The cell confluency has been determined using the Incucyte real-time video imaging system. Each data points indicate mean ± SEM. i – n Western blot analysis was carried out with specific antibodies against K10, and β-actin was used as loading control. ImageJ program was used to quantitate the protein levels. The blot is representative of three independent experiments. DD, days of differentiation
Prewounded, Single Donor, Neonatal Foreskin Tissue Derived Normal Human Epidermal Keratinocyte And Fibroblast 3d Cultures, supplied by MatTek, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/prewounded, single-donor, neonatal-foreskin tissue-derived normal human epidermal keratinocyte and fibroblast 3d cultures/product/MatTek
Average 90 stars, based on 1 article reviews
prewounded, single-donor, neonatal-foreskin tissue-derived normal human epidermal keratinocyte and fibroblast 3d cultures - by Bioz Stars, 2026-06
90/100 stars
  Buy from Supplier
human skin epidermal keratinocytes from neonatal tissue hseks  (Kurabo industries)
90
Kurabo industries human skin epidermal keratinocytes from neonatal tissue hseks
BRD4 and p63 regulates <t>keratinocytes</t> proliferation and differentiation. a, b, e, f Cell cycle analysis performed in HEKn comparing SCR condition and sip63 and siBRD4 conditions comparing untreated cells (DMSO) and JQ1 treated cells (10 µM). Cells were stained with Propidium Iodide (50 µg/ml) for 1 h and then analysed by flow cytometry. In a, e representative plot of cell cycle analysis performed in HEKn is shown, while in b, f a quantification of cell cycle analysis. Graphs present means ± SD of three independent experiments. c, g EdU-incorporation assay of HEKn cells transfected with siSCR, sip63 and different BRD4 (siBRD4#1, BRD4#2) siRNAs or treated with DMSO and JQ1 (10 µM). Data are shown as mean ± SD of N = 3 experiments. (Unpaired Student’s t test). d, h Growth curve of HEKn cells transfected with siSCR, sip63 and siBRD4#1, or treated with DMSO and JQ1 (10 µM). The cell confluency has been determined using the Incucyte real-time video imaging system. Each data points indicate mean ± SEM. i – n Western blot analysis was carried out with specific antibodies against K10, and β-actin was used as loading control. ImageJ program was used to quantitate the protein levels. The blot is representative of three independent experiments. DD, days of differentiation
Human Skin Epidermal Keratinocytes From Neonatal Tissue Hseks, supplied by Kurabo industries, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human skin epidermal keratinocytes from neonatal tissue hseks/product/Kurabo industries
Average 90 stars, based on 1 article reviews
human skin epidermal keratinocytes from neonatal tissue hseks - by Bioz Stars, 2026-06
90/100 stars
  Buy from Supplier

Image Search Results


BRD4 and p63 regulates keratinocytes proliferation and differentiation. a, b, e, f Cell cycle analysis performed in HEKn comparing SCR condition and sip63 and siBRD4 conditions comparing untreated cells (DMSO) and JQ1 treated cells (10 µM). Cells were stained with Propidium Iodide (50 µg/ml) for 1 h and then analysed by flow cytometry. In a, e representative plot of cell cycle analysis performed in HEKn is shown, while in b, f a quantification of cell cycle analysis. Graphs present means ± SD of three independent experiments. c, g EdU-incorporation assay of HEKn cells transfected with siSCR, sip63 and different BRD4 (siBRD4#1, BRD4#2) siRNAs or treated with DMSO and JQ1 (10 µM). Data are shown as mean ± SD of N = 3 experiments. (Unpaired Student’s t test). d, h Growth curve of HEKn cells transfected with siSCR, sip63 and siBRD4#1, or treated with DMSO and JQ1 (10 µM). The cell confluency has been determined using the Incucyte real-time video imaging system. Each data points indicate mean ± SEM. i – n Western blot analysis was carried out with specific antibodies against K10, and β-actin was used as loading control. ImageJ program was used to quantitate the protein levels. The blot is representative of three independent experiments. DD, days of differentiation

Journal: Biology Direct

Article Title: BRD4 sustains p63 transcriptional program in keratinocytes

doi: 10.1186/s13062-024-00547-1

Figure Lengend Snippet: BRD4 and p63 regulates keratinocytes proliferation and differentiation. a, b, e, f Cell cycle analysis performed in HEKn comparing SCR condition and sip63 and siBRD4 conditions comparing untreated cells (DMSO) and JQ1 treated cells (10 µM). Cells were stained with Propidium Iodide (50 µg/ml) for 1 h and then analysed by flow cytometry. In a, e representative plot of cell cycle analysis performed in HEKn is shown, while in b, f a quantification of cell cycle analysis. Graphs present means ± SD of three independent experiments. c, g EdU-incorporation assay of HEKn cells transfected with siSCR, sip63 and different BRD4 (siBRD4#1, BRD4#2) siRNAs or treated with DMSO and JQ1 (10 µM). Data are shown as mean ± SD of N = 3 experiments. (Unpaired Student’s t test). d, h Growth curve of HEKn cells transfected with siSCR, sip63 and siBRD4#1, or treated with DMSO and JQ1 (10 µM). The cell confluency has been determined using the Incucyte real-time video imaging system. Each data points indicate mean ± SEM. i – n Western blot analysis was carried out with specific antibodies against K10, and β-actin was used as loading control. ImageJ program was used to quantitate the protein levels. The blot is representative of three independent experiments. DD, days of differentiation

Article Snippet: Primary Normal Human Epidermal Keratinocytes, neonatal (HEKn) (Gibco, catalog no. C-001-5C) and human TERT-immortalized keratinocytes (Ker-CT) (ATCC, CRL4048, lot. no. 0213) were cultured in EpiLife medium with the addition of Human Keratinocyte Growth Supplements (HKGS, Life Technologies).

Techniques: Cell Cycle Assay, Staining, Flow Cytometry, Transfection, Imaging, Western Blot, Control

Keratinocytes lacking for p63 and BRD4 have similar expression profiles. Gene expression analysis performed using nanoString technology on HEKn cells after p63 and BRD4 silencing and JQ1 treatment. a Venn diagram represents common dysregulated genes in the different conditions. b – d Pathway gene ontology of common dysregulated genes. e – g Expression levels of the shared dysregulated genes from the top five enriched categories of the GO-pathway analysis

Journal: Biology Direct

Article Title: BRD4 sustains p63 transcriptional program in keratinocytes

doi: 10.1186/s13062-024-00547-1

Figure Lengend Snippet: Keratinocytes lacking for p63 and BRD4 have similar expression profiles. Gene expression analysis performed using nanoString technology on HEKn cells after p63 and BRD4 silencing and JQ1 treatment. a Venn diagram represents common dysregulated genes in the different conditions. b – d Pathway gene ontology of common dysregulated genes. e – g Expression levels of the shared dysregulated genes from the top five enriched categories of the GO-pathway analysis

Article Snippet: Primary Normal Human Epidermal Keratinocytes, neonatal (HEKn) (Gibco, catalog no. C-001-5C) and human TERT-immortalized keratinocytes (Ker-CT) (ATCC, CRL4048, lot. no. 0213) were cultured in EpiLife medium with the addition of Human Keratinocyte Growth Supplements (HKGS, Life Technologies).

Techniques: Expressing, Gene Expression